The goal of the research in the Little Rann of Kutch was to investigate the variety of actinomycetes that may have therapeutic significance. After gathering and processing soil samples to isolate actinomycetes, the samples were screened for antibacterial activity and carbohydrate consumption. The process of Computer-assisted identification software identification according to IDENTAX. Methicillin-resistant Staphylococcus aureus (MRSA) strains were also identified and isolated from clinical samples in this investigation. The findings demonstrated a wide range of actinomycetes species with variable carbohydrate consumption patterns and potential antibacterial action. The presence of many Streptomyces species known for their capacity to produce antibiotics was revealed by molecular identification. The study emphasizes how crucial it is to look for novel microbial resources in harsh environments, particularly in the fight against antibiotic resistance. To fully understand the bioactive substances these actinomycetes generate and their potential as future antibiotics, more study is required.