Human pathogenic fungi cause serious skin infections that persist longer. Human pathogenic fungi, namely, Aspergillus sp., was isolated from 35 years old patient suffering from fungal skin infections. Isolated fungi was identified by 18S rRNA sequencing with 1200 bp fragment. The bacteria isolated from soil sample collected from Hadapsar region of Pune, Maharashtra, India and identified as Pseudomonas stutzeri by 16S rRNA sequencing showing zone of inhibition against the isolated pathogenic Aspergillus sp. by dual culture method. The bacteria under study showed 86.66% inhibition of Aspergillus sp. This study may lead to isolation of antifungal compounds which can be utilized for treating human fungal infections.