The genus Chrysopogon is renowned for its culinary, medicinal, and aromatic uses as well as practical applications. However, research on the regeneration methods for Chrysopogon nodulibarbis (podhigar grass) remains absent to date. This study attempted to achieve a protocol for plantlet regeneration in C. nodulibarbis, initiating successful in vitro regeneration from basal shoot explants through direct organogenesis. The explants were cultured in Murashige & Skoog’s (MS) media with growth regulators such as 2,4-D, NAA, BAP, and IAA individually and also in various combinations with bovine serum albumin, charcoal, ascorbic acid and citric acid supplements. 17 distinct combinations of growth hormones were evaluated for shoot proliferation. The maximum induction frequency (90.7% and 80.4%) was noticed in BAP (3 mg/l) + NAA (3 mg/l) + Citric acid (25 mg/l) and BAP (3 mg/l) + NAA (3 mg/l) respectively. Lower induction frequencies were marked in BAP (3 mg/l), and BAP (4 mg/l) individually yielding 60.2% and 50.1%. 2,4-D (1 to 7 mg/l) and IAA (1 to 5 mg/l) of different doses did not favour callus induction. Although these are preliminary findings, further investigation is employed for fine-tuning the concentrations with the extension of sufficient growth enhancers to optimize callus induction and somatic embryogenesis.