Background: Histopathological tissue samples serve as invaluable resources for understanding the intricacies of disease processes and pathological conditions. However, the preservation of these specimens can be challenging, often leading to desiccation and irreversible damage. In this study, we explore various innovative techniques for the recovery and rehydration of intentionally dried histopathological tissue samples and provide recommendations for optimal preservation and recovery protocols for dried tissue samples in research and clinical settings. Materials and methods: The study was conducted in a tertiary health care hospital, Chennai, India. A cross-sectional observational study was conducted over a period of one year [March 2023 - March2024]. The tissue samples from various organs were intentionally dried. A standard rehydration protocol was derived based on concentration of solution and time of immersion and the tissue samples were pretreated with four different solutions before treatment with 10% neutral buffered formalin. The pretreated tissue samples were then evaluated for various parameters like color, texture and tissue adherence by naked eye examination. The histological characteristics like tissue morphology, staining quality and presence of artefacts were analyzed by microscopic examination and these features was compared with tissue samples directly treated with 10% neutral buffered formalin.