This study investigates the pharmacological activities of compounds isolated from the tubers of Eulophia herbacea Lind, focusing on their anti inflammatory and anticancer potential. The anti inflammatory activity was evaluated using a protein denaturation assay, with diclofenac sodium serving as the standard. The ethyl acetate fraction (EAF ) and the isolated compound, 1,5 dimethoxyphenanthrene 2,7 diol, demonstrated significant inhibition of protein denaturation. At a concentration of 500 μg/mL, the EAF showed 97.10% inhibition, while the isolated compound exhibited 96.79% inhibition, closel y matching the 100% inhibition observed with diclofenac sodium. The anticancer activity was assessed using the Sulforhodamine B (SRB) assay on the human lung cancer cell line (A 549). The isolated compound exhibited a dose dependent reduction in cell viabi lity, with a median growth inhibition (GI50) concentration of 14.6 μg/mL. At 80 μg/mL, the compound achieved a significant cytotoxic effect, with an average percent control growth of 32.4%, compared to 11.0% for the standard drug Adriamycin. These findings suggest that the anti inflammatory and anticancer properties of the isolated compound are substantial, with potential therapeutic applications. The study emphasizes the importance of isolating and characterizing bioactive compounds from medicinal plants t o enhance their therapeutic efficacy. Further research, including in vivo studies and clinical trials, is needed to fully understand the mechanisms underlying these effects and to validate the safety and efficacy of 1,5 dimethoxyphenanthrene 2,7 diol in hu mans. This compound represents a promising candidate for the development of new anti inflammatory and anticancer therapies.