The Marine environment is a pivotal resource for bioprospecting therapeutically active natural compounds. Molluscs are known to harbour compounds with antimicrobial, antitumor and antioxidant activities. The aim of current investigation was to evaluate the antimicrobial, anticancer, and antioxidant activities of the crude whole body methanol extract of Planaxissulcatus. FTIR was employed to identify the types of chemical bonds and functional groups present in the extract. Antimicrobial activity was assessed using Agar well diffusion assay, and determination of MIC, MBC and MFC of the extract by micro broth dilution approach. Cytotoxic effect of the tissue extract was assessed using MTT assay. Antioxidant attributes was evaluated by DPPH, Ferrous-Ion Chelating, and Nitric oxide scavenging assays. Outcomes ofthe FTIR analysis of the tissue extract has indicating the occurrence of diverse functional groups in the analyte. The gastropod extract demonstrated noteworthy antibacterial activity and antifungal activity. The minimum inhibitory concentration and minimum bactericidal and fungicidal concentrations was ranging from 0.03125- 0.156 mg/mL against tested bacteria and for Candida albicans it was 1 mg/mL.The IC50 value obtained for cytotoxicity of the tissue extract by MTT assay was showed 53.99 μg/mL. Antioxidant assays demonstrated IC50 value of 565.80, 770.53 and, 758.7 μg/mL for DPPH assay, Ferrous-Ion Chelating assay, and Nitric oxide scavengingassay The outcome of the present study confirmed the presence of bioactive compounds. Decisively, the bioactive compounds extracted from P. sulcatus have significant potential to be employed as therapeutic agents.