This research examines the antibacterial properties of Ethanolic extracts derived from several components of Alstonia scholaris, such as leaves, follicles, and latex. The antibacterial effectiveness was assessed against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli. Out of all the extracts, the latex showed the most antibacterial activity, specifically against B. subtilis. It created a zone of inhibition measuring 21 mm when tested at a concentration of 100 mg/ml. The extracts obtained from leaves and follicles also shown notable antibacterial activity, but to a lower degree. The efficacy of the extracts was less prominent against P. aeruginosa, suggesting a specific bactericidal activity that is more powerful against Gram-positive bacteria. The antioxidant capacity was assessed via the use of DPPH free radical and superoxide anion scavenging tests. The latex extract had the greatest antioxidant activity, with a DPPH scavenging rate of 52% at a concentration of 100 mg/ml and a superoxide anion scavenging rate of 55% at a concentration of 15 mg/ml. The leaf extracts exhibited significant antioxidant capability, but the follicle extracts shown modest action. The results indicate that the latex of A. scholaris has considerable promise for antibacterial and antioxidant uses, providing a natural resource for the development of medicinal substances. The structural analysis of Alstonia scholaris was conducted using HPLC and a range of spectroscopic techniques. This analysis verified the presence of certain methyl and methylene groups, which are characteristic of a lactone ring. This research highlights the favourable bioactive characteristics of A. scholaris, namely its latex, and confirms its potential use in pharmaceutical formulations.