This study investigates the phytochemical constituents and antioxidant activity of Morus alba leaves extract. The ethanolic extract of Morus alba yielded 8.2% (w/w), and phytochemical screening confirmed the presence of flavonoids, phenols, and sterols. These constituents were identified through positive reactions in tests such as lead acetate, alkaline reagent, ferric chloride, and Salkowski's tests. The antioxidant potential of the Morus alba extract was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and compared with ascorbic acid as a standard antioxidant. The extract demonstrated concentration-dependent antioxidant activity, with an IC50 value of 50.85 µg/ml, indicating its ability to scavenge free radicals and neutralize oxidative stress. Ascorbic acid, used as a positive control, exhibited a lower IC50 value of 24.92 µg/ml, suggesting higher antioxidant potency compared to the Morus alba extract. In vitro assays showed that the Morus alba extract achieved a maximum inhibition of 83.78% of DPPH radicals at a concentration of 100 µg/ml. The extract's antioxidant activity is attributed to its phytochemical composition, particularly flavonoids and phenols. These findings suggest that Morus alba extract could serve as a natural antioxidant source with potential applications in combating oxidative stress-related disorders. Further studies are needed to explore its efficacy in vivo and elucidate its mechanisms of action.