Bifonazole-loaded transethosomes represent a novel approach in topical drug delivery for treating fungal infections. This study aimed to develop and characterize transethosomal formulations of bifonazole, focusing on vesicle size, entrapment efficiency, pH, viscosity, drug release kinetics, and morphology. The optimized formulation (F3) exhibited a vesicle size of 111.45 nm with 70.67% entrapment efficiency. Transethosomal gels maintained pH values (6.3-6.9) suitable for skin application, with viscosity ranging from 2585 to 3895 cp, ensuring ease of application. In vitro release studies demonstrated sustained drug release profiles, with TEG3 releasing 89.23% of bifonazole over 24 hours, significantly higher than conventional gels (33.44%). Microscopic examination confirmed spherical vesicles, ensuring uniform drug delivery and skin penetration. This study highlights the potential of bifonazole-loaded transethosomes to enhance topical fungal infection treatment through sustained drug release and improved skin penetration.