This study aimed to validate the hepatoprotective activity of extracts from Naregamia alata against Galactosamine-induced hepatotoxicity in HepG2 cells. Cells were exposed to different extracts at the concentrations of (1.56 μg/ml – 12.5 μg/ml) in combination with Galactosamine (0.1%) for 24 hours. Hepatoprotective effects were assessed through various assays, including cell proliferation and oxidative stress mechanism. The anti-proliferative effect was improved in the presence of all the plant extracts at all the tested concentrations. Ethylacetate extract treated HepG2 cells were 70.14% reduction in Galactosamine induced group. Galactosamine led to 198.25% increase in MDA level, which was attenuated by Ethylacetate extract at 138.37% at 6.25 ug/ml. GSH content in cells decreased by 52.25% with Galactosamine, but the plant extract increased GSH levels at 73.25%. Additionally, Galactosamine induced a 34.26 decrease in SOD level, but this necrotic effect was diminished by treatment group 71.85%. In conclusion, extracts from Naregamia alata demonstrated a preventive effect on HepG2 cell injury induced by Galactosamine treatment for 24 hours. The hepatoprotective activity of Ethylacetate extract was also comparable with that of the standard drug silymarin.