Deoxyribonucleic acid (DNA) itself is a chain of nucleotides. Under most conditions, DNA is double-stranded, consisting of two such nucleotide chains that wind around each other in the famous shape known as the double helix. Staphylococcus aureus is a versatile and virulent pathogen in humans, who serve as natural reservoirs for this pathogen. DNA-based methods for detecting foodborne bacterial pathogens usually result from extracting DNA from samples without enrichment. Physiological and mechanical barriers to the isolation of DNA from complex organic material may occur. This experiment aimed to visualize and amplify the extracted DNA from Staphylococcus aureus using Polymerase chain reaction (PCR) and gel electrophoresis. Agarose gel electrophoresis is routinely used to separate proteins, DNA or RNA. The objective of the PCR is to produce a large amount of DNA in a test tube, starting from only a trace amount. The experiment was conducted at the biotechnology laboratory of (Devkota et al. (2012) at West Visayas State University, La Paz, Iloilo City, on May 9, 2019. The agarose gel electrophoresis result indicated a successful amplification of the desired DNA fragment during the Polymerase Chain Reaction (PCR) with gene primer sizes of 1.5 to 2.0bp.