This study explores the ampelographic characteristics and physiological performance of five previously undescribed autochthonous grapevine varieties from the Aures region of Algeria. A comprehensive morphological analysis of 15 ampelometric parameters revealed significant differences between the varieties, with leaf sinus depth, vein angles, and leaf size emerging as key distinguishing factors. Hierarchical clustering grouped the varieties into two main clusters, highlighting similarities between Anonymous 3, 5, and 1, and distinct characteristics in Anonymous 2 and 4. Physiological assessments focused on rooting ability, acclimatization success, and shoot growth dynamics. The results showed varying rooting rates (30 % to 90 %), root lengths (3.8 cm to 6.5 cm), and acclimatization rates (33 % to 78 %), indicating diverse physiological responses. Shoot growth analysis revealed distinct growth patterns, with Anonymous 5 exhibiting the most vigorous growth (17.0 cm) and Anonymous 4 showing the slowest growth (11.5 cm). Notably, initial rooting success did not consistently predict subsequent growth performance, suggesting complex interactions between genetic and physiological factors during early grapevine development. These findings have important implications for optimizing propagation techniques and early vineyard management practices for these unique varieties, while contributing to the conservation and potential utilization of autochthonous grapevine genetic resources.