The basic objective of this study was to establish a green assessment straightforward method to use, economical UV spectroscopic method for the estimation of dosage formulations for tofacitinib tablets. The recommended approach employs a solvent mixture of ethanol and water (20:80), with the detection wavelength at 293 nm. It is highly specific, showing no interference with blank. The method demonstrates linearity within the spectrum of concentration of 2.00 to 14.01 mcg/mL, with a remarkable coefficient of correlation value is 0.999. Precision is confirmed with RSD below 2 % and % recovery of drug found to be 99.55 to 99.84, while the limits of quantification and detection are determined as 0.515 µg/mL and 1.562 µg/mL accordingly. Moreover, the solution remains stable for 24 hours under room temperature. Validation in accordance with ICH Q2(R1) requirements, were shown to be satisfactory with regard to the regular analysis of tofacitinib in tofacitinib tablets by UV spectroscopy. Moreover, the environmental friendliness of the developed methods—which rely on solvents like ethanol and water—was underlined by the greenness assessment carried out using the AGREE software. These results support replacing currently used procedures for the determination of tofacitinib in tablet formulation with our environmentally friendly and analyst-friendly approaches.