Nanotechnology is an expanding field of research that has many practical uses across multiple scientific and technological areas, including biomedicine, agriculture, pharmaceutics, textile manufacturing, food technology, and catalysis. The procedure of green nanoparticle synthesis involves the use of plant extracts as reducing, capping, and stabilizing agents, replacing the need for costly and hazardous chemicals. The objective of this study was to produce and analyseCuO nanoparticles using Carica papaya methanolic leaf extracts in order to assess their antibacterial and antioxidant properties. The characterization of CuO-NP revealed that the synthesised nanoparticles had a greater degree of light absorption at a wavelength of 550 nm. Additionally, the SEM analysis showed that the CuO-NPs had a spherical structure with diameters ranging from 26.8 to 38.7 μm. Furthermore, the XRD data revealed that the average particle sizes of the CuO-NPs were 17 nm. At a dosage of 10 mg/mL, the CuO-NPs demonstrated the highest level of antibacterial activity against all tested microbes. The DPPH, superoxide radical scavenging activities, and FRAP activities of CuO-NPs exhibited a significant increase (<.0005) as the extract concentrations increased. In addition, the CuO-NP exhibits a significant positive correlation (r = 0.9702) with increasing concentrations for DPPH, superoxide radical scavenging activities, and FRAP activities. Similarly, the correlation coefficients for superoxide radical scavenging activities and FRAP activities are r=0.9496 and r=0.982, respectively. This study suggests that CuO-NPs have potential antibacterial and antioxidant characteristics, exhibiting free radical scavenging actions that can protect against oxidative stress and microbial infections.