The green synthesis of pyrazoline derivatives with possible antibacterial, anti-inflammatory, and antioxidant effects is investigated in this work. The goal of the research was to create efficient and sustainable pyrazoline synthesis routes by utilizing environmentally friendly techniques including microwave irradiation and grinding-based technologies. The DPPH radical scavenging method was used to evaluate the antioxidant activity of the synthesized pyrazolines. The results showed considerable radical scavenging capabilities, with compounds 3a, 3d, and 3e exhibiting the maximum activity comparable to ascorbic acid. Using the HRBC membrane stabilization method, anti-inflammatory capabilities were assessed. Compounds 3c, 3d, and 3e showed strong antiinflammatory effects that were comparable to aspirin. Furthermore, pyrazoline derivative 3b showed the strongest antibacterial activity against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli, according to antimicrobial screening results. Other derivatives also indicated noteworthy performance. Overall, the study indicates that these pyrazoline derivatives have intriguing biological properties and may be further developed for medicinal purposes.