This study focuses on the pre-formulation assessment of Mesalamine, an established anti-inflammatory drug, and Kaempferol, a natural flavonoid with potential therapeutic benefits. The aim is to concurrently estimate the two compounds using advanced analytical techniques. The research encompasses a thorough investigation of the physicochemical properties, compatibility, and stability of Mesalamine and Kaempferol. The simultaneous estimation of these compounds is crucial for the development of combination formulations, where their synergistic effects can be harnessed for enhanced therapeutic outcomes. A novel, simple, sensitive and rapid spectrophotometric method has been developed for simultaneous estimation of Mesalamine and Kaempferol. The method involved solving simultaneous equations based on measurement of absorbance at two wavelengths, 303 nm and 365 nm, λ max of Mesalamine and Kaempferol respectively. Beer's law was obeyed in the concentration range of 5-35 μg/ml and 10-60 μg/ml for Mesalamine and Kaempferol respectively. The method was found to be precise, accurate, and specific. The proposed method was successfully applied for estimation of Mesalamine and Kaempferol combinely. This research contributes to the pharmaceutical field by providing valuable insights into the pre-formulation aspects and simultaneous estimation of Mesalamine and Kaempferol, paving the way for the development of pharmaceutical formulations and to serves as a foundation for optimizing drug combinations and potentially improving treatment efficacy