The Yamuna River is one of the major tributaries of the river Ganga originated in the Himalayas and flowing through a varied geological terrain. The present study was focused on heavy metal analysis in different regions of Delhi including rural, semi-rural and urban areas. The water sample was taken from six different locations and analyzed for pH, EC, BOD, DO along with heavy metal analysis. The results show that the Yamuna River water is alkaline in nature and the dissolved oxygen concentration is within the permissible limit except in the Nizamuddin area. The BOD was found to vary from 12.4 to 124.5mg/l which was higher than the permissible limit. Heavy metal analysis shows that Fe, Cu, Cr, and Zn lie below their permissible limit while Ni, CN, Pb and Co lies above the permissible limit of BIS. The present work highlights the pollution load of heavy metals in the river Yamuna and also advocates an urgent attention towards minimizing the health risk of people residing not only along the riverbanks and surrounding regions but also for city population.