Background: Silver nanoparticles (AgNPs) are widely used in biomedical and industrial applications due to their unique antimicrobial properties. Despite their extensive utility, the potential toxicological risks, especially during pregnancy, necessitate a comprehensive evaluation. Objective: This study aims to investigate the effects of biodegradable synthesized AgNP colloidal solutions administered via oral gavages on the biochemical parameters, oxidative stress markers, and cellular integrity in pregnant Swiss albino mice and their fetuses. Methods: Pregnant Swiss albino mice were treated with different concentrations of AgNPs. Biochemical parameters in the mother's blood serum and fetal tissues were quantified, including glucose, calcium, protein, creatinine, total bilirubin levels, catalase activity, and reduced glutathione activity. The study employed a detailed methodology for assessing oxidative stress and potential toxicological mechanisms. Results: Biochemical Changes: Significant decreases in maternal blood serum glucose and calcium levels were observed post-AgNP treatment, alongside dose-dependent alterations in protein, creatinine, and total bilirubin levels. Oxidative Stress Indicators: Liver catalase activity decreased with increasing AgNP doses, indicating enhanced oxidative stress. Similarly, reduced glutathione activity in brain tissue significantly decreased with dose increments.