A medicinal plant is one that has compounds that can be utilized therapeutically or that serve as building blocks for the production of effective pharmaceutical products. The antibacterial activity of Psoralea corylifolia in seed and leaf extracts was proven in Pseudomonas sp., E. coli, and staphylococcus species, but no researcher was able to observe the antimicrobial activity in many additional gram positive and gram negative bacteria. Thus, the present research work was aimed at identification of the antimicrobial activity of Psoralea corylifolia seed extract in four different solvents viz., chloroform, acetone, ethanol and water. Five-gram (+ve) bacteria namely B-megatrium, Sarcina lutea, β-haemolyticus, Streptococcus andM. tuberculosis and five-gram (-ve) bacteria namely S. shiga, S. boydii, S. flexneri, S. sonnei and S. sarcinaceae were selected for the study. Ciprofloxacin and Vancomycin were used as antibiotics against gram (+ve) and gram (-ve) bacteria. The ethanol extract showed the highest zone of inhibition against S. flexneri at 16 nm, which was very close to the standard antibiotic's zone of inhibition (18 mm). Acetone, water, and ethanol were next with zones of inhibition of 15 nm against S. shiga, S. sonnei, and S. sarcinaceae, respectively. Aqueous extract against Streptococcus had a zone of inhibition of 21 nm, which was followed by ethanol extract against S. haemolyticus, which had a zone of inhibition of 22 nm. This was significantly closer to the usual zone of inhibition of the chosen antibiotic, ciprofloxacin. In conclusion, Psoralea corylifolia L. ethanolic extracts may be a source of fresh, potent herbal medications for treating infections brought on by multi-drug resistance strains of bacteria in both community and hospital settings.