Urinary tract infections (UTIs) are a major worldwide health issue, especially among women, and Uropathogenic Escherichia coli (E. coli) is the most common causal agent. However, the effectiveness of traditional therapies, such as nitrofurantoin, is hampered by antibiotic resistance, exacerbated by premature medication discontinuation. This study examines the possibility of Uroteuthis edulis (Squid) ink extract as an alternative therapy option for UTIs. The study used quantitative experimental approaches to establish squid ink's potential antibacterial activity against Escherichia coli and Staphylococcus saprophyticus. The findings highlight the untapped potential of marine-derived compounds in addressing the growing challenge of antibiotic resistance in UTI treatment paradigms.