Objective: The aim of the current study was to assess the effect of Corchorus Olitorius on Diabetes Mellitus induced by Streptozotocin (STZ) in experimental rats and how Corchorus Olitorius helps to mitigate the effects. Methods: The leafs of Corchorus Olitorius was extracted using hot extraction method by using solvent like n-hexane, ethyl acetate, methanol and phytochemical screening was carried out. Wistar Albino rats of weight 120-130 grams were divided into 6 groups- normal, control, standard, treatment I, treatment II, treatment III with various dose and each group containing 5 animals. In-vitro screening methods were carried out to evaluate the various properties of Corchorus Olitorius. Results: Methanolic extract of C.olitorius shows maximum inhibition rather than ethyl acetate and n-hexane extract of C.olitorius. Conclusion: Collection, authentication & identification of C.olitorius was done and also successive extraction, phytochemical screening and in-vitro studies like DPPH, Alpha glucosidase activity and alpha amylase activity.