Introduction Regeneration of the periodontal tissues is a challenging endeavor due to the complex anatomy and function of these structures. Cissus quadrangularis (CQ), a medicinal plant with known anti-inflammatory and osteogenic properties, has shown promising results in various tissue engineering applications. There has been growing interest in utilizing bioactive materials and advanced techniques to enhance periodontal regeneration.Therefore, the aim of the study is to fabricate and evaluate the novel cissus quadrangularis infused with platelet rich fibrin and methacrylated hyaluronic acid based scaffold for periodontal regeneration.