Duranta erecta, a widely distributed medicinal plant, has garnered attention for its diverse pharmacological properties. This study investigates the in vivo and in vitro activities of Duranta erecta silver nanoparticles (DE-AgNPs). The antimicrobial efficacy of (DE-AgNPs) was assessed through in vitro studies against spectrum of pathogenic microorganisms. Results demonstrated notable inhibitory effects on bacterial strains, indicating the promising antimicrobial activity of DE-AgNPs. In vivo investigations were conducted to evaluate the anti-inflammatory potential of DE-AgNPs using relevant animal models. The results exhibited significant reductions in inflammatory markers, suggesting the nanoparticles' ability to mitigate inflammatory responses. Furthermore, the study explored the efficacy of DEAgNPs in a peritonitis model, revealing their potential in managing this lifethreatening condition. The findings of this research highlight Duranta erecta silver nanoparticles as a multifaceted therapeutic agent with promising antimicrobial, anti-inflammatory, and peritonitis management properties. These results contribute valuable insights into the potential applications of nanotechnology-derived formulations from medicinal plants, paving the way for further exploration and development of novel therapeutic interventions