Staphylococcus aureus is the common cause for osteomyelitis in adults and children, that can be treated by antibacterial agents. Resistance development on continuous exposure to antibacterial agents, is the motivation to find some new antibacterial agents from synthetic and natural source. This research was aimed to determine the antibacterial activity of new sesamol derivative and biosynthesized silver nanoparticles against S. aureus (the osteomyelitis causing bacteria). Study involved synthesis of new imino derivative of sesamol (IDS) and silver nanoparticles of sesamol rich Sesame seed oil. Current investigation involved synthesis of IDS by esterification of sesamol followed by hydrazination and Schiff’s reaction. This study also involved biosynthesis of silver nanoparticles of Sesame seed oil (SNPSSO) using AgNO3. The synthesized IDS and biosynthesized SNPSSO was characterized using IR, NMR & MASS spectrometry, and UV-Vis. Spectrometry respectively. The synthesized IDS and SNPSSO were further evaluated for their antibacterial potential against S. aureus using disk diffusion method. Study revealed high antibacterial potential of IDS when compared to SNPSSO. Although, current study reports the new IDS as promising antibacterial agents for infectious osteomyelitis, however the new IDS should be further evaluated for its preclinical and clinical significance.