This research investigates innovative biocontrol strategies for managing root-knot nematodes (Meloidogyne spp.) in cucumber crops by employing fungal isolates from Pochonia and Lecanicillium species. The study, conducted in a controlled greenhouse environment, focuses on the effectiveness of isolates IRAN 3222 C (Lecanicillium) and IRAN 457 C (Pochonia) in mitigating nematode-induced damage. The findings reveal that Lecanicillium isolates provided moderate control of nematode populations, leading to improved plant vitality. However, Pochonia isolates exhibited a stronger biocontrol effect, significantly nematode reproduction and enhancing overall plant health. This work highlights the potential of Pochonia and Lecanicillium fungi as sustainable alternatives to chemical treatments in nematode management, promoting healthier crop production practices.