Recently, herbal products and supplements have beenused tremendously for health care.Propolis, a byproduct secreted by honey beesfor protecting the hive from fungal infections is used in our current study to identify the biochemical compounds by GCMS analysis and to evaluate the antidiabetic activity of Propolis. Aqueous and phenolic extracts of Propolis were selected for the enzyme inhibition activity against alpha-amylase and alpha-glucosidase. GCMS analysis fetches the presence of forty various compounds and among thosea few compounds possess high biological activities like Hexadecanoic acid, Dinaphtho[2,1-b:1',2'-d]furan and Chrysin. These compounds were reported to possess different therapeutic effects from earlier studies. In vitro α-amylase and α- Glucosidase activity was carried out with the help of aqueous and phenolic extract of propolis andthe percentage inhibition of assays showed maximum inhibitory effects were Phenol extract of Propolis against alpha-amylase inhibited 7.62%, 13.2%, 24.5%, 38.5 % and 49.9% respectively. In the same way, the aqueous extract of propolis inhibits alpha-amylase8.7%, 15.7%, 29.3%, 48.7% and 61.7% in various concentrations. respectively. Similarly, the phenolic and aqueous extracts reacted against alpha-glucosidase in various concentrations the inhibitory results are 8.6%, 15.0%, 27.7%, 44.3 % and 51.3% respectively. In the same way, the aqueous extract of propolis shows 10.7%, 18.5%, 37.3%, 56.0% and 67.7% respectively.In vitro α-amylase and α- Glucosidase activity has shownthat the aqueous propolis extract exhibits a higher inhibitory effect when compared to that of phenolic extract.This study suggests the aqueousextract of Propolis might be considered as a potential source of bioactive constituents with excellent antidiabetic activity.