The current study was aimed to evaluate the 50% hydroethanolic Barleria cristata L. stem extract's in vitro free radical scavenging, hypoglycemic, and hypolipidemic potential in diabetic rats induced by alloxan. Presence of alkaloids, flavonoids, glycosides, phenols, saponins, tannins, carbohydrates, and protein were determined by phytochemical analysis.The plant extract's physiochemical composition supported its long-term storage. Better DPPH, H2O2, NO, and SO radical scavenging activity of plant extract supported its antioxidant potential. The plant extract did not exhibit any negative effects during the course of the trial in an acute toxicity study. Alloxan monohydrate was used to induce diabetes in rats. Glibenclamide serving as the usual standard treatment. 200 and 400 mg/kg b.w. of 50% hydroethanolic stem extract treatment is given to diabetic Wister albino rats. In diabetics, the fasting blood glucose, gHB, urea, creatinine, glucose 6 phosphatase, MDA, TC, TG, LDL, and VLDL levels were decreased. In plant extracts treated rats, levels of glycogen, insulin, body weight, and HDA were elevated. It was also discovered that transaminase activity had been restored. This study demonstrates that plant extracts include phytocomponents with potential for lowering blood sugar and cholesterol that can be taken as supplements to treat diabetes.