The aimof this study is to characterize the isolated compounds from herbal extracts of two Indian medicinal plants namely Aerva lanata(Family: Amaranthaceae) and Curcuma caesia(Family: Zingiberaceae).For the purpose of isolating alkaloids as an active component from Aerva lanata roots and phenolic compounds from Curcuma caesia rhizomes, a basified toluene sub-fraction was optimised. Using column chromatography and preparative TLC, both were extracted. The phytochemical analysis of both the plants has been previously reported in my research publication.Using instrumental analytical techniques, the chemicals were extracted from both plants and characterised. TLC profile of successive extracts and basified fractions of Aerva lanata roots confirmed the presence of strong alkaloids at 0.45 and 0.54 Rf value (in successively basified toluene extract), while flavanoids at 0.55 Rf value were detected in the rhizomes of Curcuma caesia (in successively basified ethyl acetate extract). Both of the isolated compounds—alkaloids from Aerva lanata named IAL and flavanoids from Curcuma caesia named ICC—were identified as polyphenol derivatives and canthine-6-one derivatives, respectively, using UV-Vis, IR, 1HNMR, and mass spectrometry.According to the study mentioned above, both plants contain a variety of phytoconstituents, including carbohydrates, phenolics, alkaloids, flavonoids, and phytosterols, which