Three sites were selected in Sidi Bel Abbes province (Algeria’s northwest) to isolate Corynebacterium species, due to their organic matter content. Four isolates were selected according to their morphology, microscopy and biochemical tests according to Bergey’s manual that they are Corynebacterium. Then, the isolates were cultivated in two media Nutrient Broth and Brain Heart Infusion at 37°C to determine the natural growth rate. S5 exhibited the highest growth rate μ=0.182h-1 in NB, and μ=0.224h-1 in BHI, indicating a 23.1% improvement. BHI’s complex nutrient composition (including proteins, and other growth factors) likely favors fast-growing isolates like S4 and S5, which may possess more robust or efficient biosynthetic pathways, compared to the simpler nutrient composition of NB may be a limiting factor for all isolates. Finally, three media were used to produce glutamic acid because it is the most produced and sold worldwide: D1 (glucose as the sole carbon source), D2 (fructose as the sole carbon source), and F1 (both). S4 exhibited the highest glutamic acid production, 7.2 g/l in D1 at pH 7.5 and 30°C. Generally, the lower the initial pH of the medium, the lower the glutamic acid production will be, and the optimum temperature was 30°C compared to 37°C.