A soil sample (T42.2) was collected from a rhizosphere of Ocimum tenuiflorum (tulsi) of Maharashtra, India and diluted to 10-2 after which, it was spread on Glycerol Aspargine Agar. The colony with actinobacteria like morpholoigical characters represented thread like structures with the cells attached to each others with a Gram Positive nature. Biochemical characters were catalase negative, starch and Triple Sugar Iron utilization positive. The culture was then processed for molecular identification based on their partial sequences of 16S ribosomal RNA genes which was identified as Streptomyces rochei. It showed 98.45% identity with partial 16S ribosomal rRNA gene sequence of Streptomyces rochei strain NRRI B-2410. Its evolutionary analysis was performed with Maximum Parsimony Method by constructing a phylogenetic tree showing its closest clade with JF304944.1:3-769 Streptomyces sp. BN-6. The study revealed that Streptomyces rochei has a habitat in the rhizosphere soil of Ocimum tenuiflorum (tulsi) for its further medical applications. Further, the study showed the need of increased species level identification data of Streptomyces genus in NCBI database to prevent misidentifications