Background: Breast cancer is a significant public health concern globally, with early detection being crucial for improving outcomes. However, there is limited understanding of Breast SelfExamination (BSE) knowledge, perception, and practice among female Saudi nursing students. Methods: A cross-sectional study was conducted to investigate the knowledge, perception, and practice of BSE among female Saudi nursing students. Data were collected using a structured survey instrument and analyzed to identify patterns and associations. Results: Participants demonstrated high awareness of breast cancer signs, with notable recognition of breast lumps, changes in breast size or shape, nipple discharge, and skin changes. Although 93.3% reported knowledge of BSE, only 78.0% practiced it at least once, with intervals ranging from months (53.3%) to years (11.2%). Participants showed varied levels of self-perceived competency in performing BSE, with 36.7% rating themselves highly competent and 6.1% not at all competent. Factors influencing BSE practice included academic progression, GPA, health status, family history of breast cancer, and education about breast cancer and BSE. Participants who rated themselves as highly competent in BSE were more likely to practice it regularly.