A One-pot multicomponent synthesis of 2, 4, 5 triaryl-1H Imidazoles using meglumine sulphate as a catalyst with involves reaction of benzil, substituted aromatic aldehyde and ammonium acetate under reflux in ethanol solvent conditions, synthesised compounds have been categorized through spectroscopic techniques like IR, 1HNMR and Mass spectra, the prepared compounds were nontoxic, excellent yields. The remarkable advantage of this method is a low-cost eco-friendliness catalyst, rapidly completion of reaction and few volumes of using organic solvent, excellent yield and mild condition. Additionally, all synthesized compounds were screened for antimicrobial and antifungal activities.