Allium fistulosum, a widely cultivated perennial plant in Asia, has a rich history of traditional medicinal use. This study aims to comprehensively characterize the metabolite profile of A. fistulosum ethanol extract using GC-MS analysis, evaluate its antioxidant potential, and perform molecular docking studies with identified compounds. A. fistulosum ethanol extract exhibited significant antioxidant activity with IC50 values of 63.12 μg/ml (NORSA), 72.79 μg/ml (ABTS), 78.35 μg/ml (DPPH), and 47.45 μg/ml (HRSA). Phytochemical analysis revealed phenolic compounds (1.2 mg/g), flavonoids (0.86 mg/g), phytosterols (3.3 mg/g), tannins (5.12 mg/g), total steroids (11.2 mg/g), protein (13.6 mg/g), sugar (0.86 mg/g), and lipids (21.8 mg/g). The compound 3 (1,3-benzenedicarboxylic acid, bis(2-ethylhexyl) ester) identified through GC-MS analysis were utilized for molecular docking studies, revealing a substantial binding affinity of -7.4 kcal/mol. A. fistulosum ethanol extract exhibited significant antioxidant activity with IC50 values. This research sets the stage for further investigations into the development of targeted therapies for cancer treatment.