Although it is widely acknowledged that tobacco smoke includes carcinogens, there is some disagreement in the scientific literature on the relationship between smoking habits and the incidence of micronuclei. In the Tamil Nadu district of Dharmapuri, a study was conducted to evaluate and compare the prevalence of micronuclei in long-term beedi smokers and non-smokers. Of the subjects, 70 were long-term smokers of beedi, and the remaining 70 did not smoke. The average age of the controls was 46.38±5.3, while the smokers' average age was 48.77±6.5. There was a significant relationship in both the subjects and the controls between the frequency of micronucleated cells and tobacco use, as well as between radiation exposure and the presence of micronuclei (p>0.05). Based on the frequency of habit and age, subjects who regularly smoked tendu leaves had a greater incidence of MN within the smokers group. While smoking is a significant factor in the creation of micronuclei, other factors that affect their frequency include ionising radiation and personal habits. In addition, cytogenetic modifications such pyknosis and karyolysis can be included in future studies to increase the specificity