Purpose: Urinary lithiasis is globally prevalent irrespective of gender and age. Now, it is a vital step to find out the mineral composition of kidney stones along with the ins and outs of this disease to develop prophylactic measures. The present work represents identifying minerals and their composition of 30 renal calculi collected from patients suffering with kidney stones formation with the aid of infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). Patients & Methods: Prominent pioneering analytical techniques were employed to 30 renal calculi sample collected through nephrectomy or percutaneous nephrolithotomy surgical procedures. They were studied for biochemical and spectroscopic analysis. All the 30 samples were investigated for ammonia, carbonate, oxalate, calcium and phosphate, uric acid, cystine, magnesium followed by FT-IR, XRD and SEM analysis.