This study represents an initial investigation into the protective potential of phenolic compounds hesperetin, genistein, and gingerol against toxicity triggered by elevated glucose concentration in pancreatic β cells in vitro. Pancreatic β cells (RIN-5F) were grown under normal (5mM) and high (25mM) glucose concentrations, and the cell viability were assessed. Glucose stimulated insulin secretion was also measured after treatment of cells for 24 hr under normal and high glucose concentration and then treatment with phenolic compounds.