HPTLC fingerprints have attained a vital role towards automated biometric authentication of primary and secondary metabolites in herbal extract. Today, worldwide researchers are in mutual agreement that the biological and medicinal property of Curcuma longa Linn are primarily attributed to its curcuminoids (a polyphenolic combination of secondary metabolites i.e. curcumin, demethoxy curcumin, and bisdemethoxycurcumin). This is a study aimed to validate the authenticity of bisdemethoxycurcumin (BDMC) in solvent-evaporated dried extracts of Curcuma longa Linn (CE) through HPTLC fingerprints. HPTLC bands were scanned and visualized at shorter (254 nm) to longer (366 nm) wavelengths of UV radiation and in white light. The chromatograms of HPTLC amply demonstrated the presence of BDMC (a bioactive detoxifier and immunomodulator) in the hydroethanolic extract of Curcuma longa Linn (CE). This contextual study will emphasize the significance of HPTLC to trace specific metabolites in herbal sample.