Nanostructured lipid carriers (NLCs) have provoked the incessant impulsion for the development of safe and valuable drug delivery systems owing to their exceptional physicochemical and then biocompatible characteristics. Lipid nanocarriers are developed as an alternative to polymeric nanoparticles, liposomes and emulsions. Further, Nanostructured Lipid Nanocarriers are the second generation lipid carriers developed to overcome problems associated with Solid Lipid Nanoparticles and are utilized in various therapeutic approaches. In situ gelling systems becomes one of the most popular and prominent. It had a tremendous potential advantage of delivery systems due to many benefits like easy-to-use simple manufacturing; improve both adherence and patient comfort by minimizing the frequency of drug administration by its unique characteristics feature of sol to gel transition Mucormycosis is an infection caused by a group of filamentous molds within the order Mucorales. Infections may result from ingestion of contaminated food, inhalation of spores into the nares or lungs, or inoculation into disrupted skin or wounds. In developed countries, mucormycosis occurs primarily in severely immunocompromised hosts. This article describes the NLC with respect to structures, methods of preparation, advantages and its application of NLCs over first generation lipid nanoparticles and Mucormycosis with respect to their type of Mucormycosis, scenario, overview, the current treatment of Mucormycosis.