An investigation analyzed the proximate composition of two Cyprinid species commonly consumed by the Khynriam sub-tribe of the Khasi community: Neolissocheilus hexagonolepis (Khasaw) and Pethia shalynius (Shalynnai). Measurements included protein, total free amino acid, carbohydrate, lipid, pH, and trace elements. The results showed significant differences (P<0.05) between the two species. Protein content was 0.9144 (±0.0508) in Neolissocheilus hexagonolepis and 1.944 (±0.181) in Pethia shalynius. Amino acid content was 12.82 (±1.84) in Neolissocheilus hexagonolepis and 49.03 (±5.44) in Pethia shalynius. Carbohydrate content was 0.1406 (±0.423) in Neolissocheilus hexagonolepis and 0.0870 (±0.0223) in Pethia shalynius. Lipid content was 4.333 (±0.577) in Neolissocheilus hexagonolepis and 7.333 (±0.577) in Pethia shalynius. Both species had slightly acidic pH values: 6.667 (±0.115) for Neolissocheilus hexagonolepis and 6.967 (±0.153) for Pethia shalynius. Essential minerals Fe, Zn, and Cu were higher in Pethia shalynius, while Mn was slightly higher in Neolissocheilus hexagonolepis. Zn content was highest in Neolissocheilus hexagonolepis, followed by Mn, Fe, and Cu. In Pethia shalynius, Fe content was highest, followed by Mn, Zn, and Cu. These findings provide valuable insights into the nutritional value of the two fish species, both of which are recommended for daily consumption to ensure a balanced diet. Encouraging the consumption of both species could enhance the nutritional diet of the Khynriam sub-tribe of the Khasi community.