The emergence of Methicillin-resistant Staphylococcus aureus (MRSA) as a formidable pathogen necessitates the discovery of novel antibiotics. Streptomyces geysiriensis lk8, isolated from the saline desert soil of Kutchha, exhibits promising anti-MRSA activity. This study aims to optimize the cultural and environmental conditions for maximizing the anti-MRSA potential of this strain. Initial screening identified significant antibacterial activity against MRSA, prompting further optimization efforts .A systematic approach was employed to enhance antibiotic production, starting with the optimization of media components. Various carbon (glucose, sucrose, starch) and nitrogen sources (peptone, yeast extract, ammonium sulfate) were tested. The influence of pH (6.0-9.0), and salinity (0%-10% NaCl) on antibiotic production was assessed. This comprehensive optimization strategy highlights the significant promise of S. geysiriensis lk8 in addressing the urgent need for effective MRSA treatments