This study investigates the physicochemical and bacteriological quality of spring water from five sources in the wilaya of Skikda, Algeria, focusing on areas in the communes of Zitouna and Kanoua. The physicochemical analyses were conducted using electrochemical and volumetric methods, while bacteriological analyses involved membrane filtration techniques. The sampling covered various parameters including pH, temperature, electrical conductivity, turbidity, total dissolved solids (TDS), alkalinity, hardness, and concentrations of calcium, magnesium, chloride, iron, copper, zinc, nitrate, nitrite, ammonium, phosphate, and sulfate. The results show that all physicochemical parameters generally meet Algerian and international standards, indicating good water quality. pH values ranged from 6.5 to 7, with temperatures below 25°C, and electrical conductivity, turbidity, and TDS levels within acceptable limits. However, total alkalinity was absent, and while most sites had calcium and magnesium concentrations within standard limits, some exceeded acceptable levels. Bacteriological analyses revealed that Sites S1, S3, and S5 complied with Algerian standards for total coliforms, fecal coliforms, fecal streptococci, and Escherichia coli, indicating safe drinking water. In contrast, Sites S2 and S4 exceeded acceptable levels for total and fecal coliforms, suggesting significant contamination issues. Overall, the study underscores the importance of regular monitoring and targeted interventions to address water quality issues, particularly in sites with higher contamination levels. Implementing effective water treatment and management practices is essential to ensure consistent compliance with safety standards and protect public health.