In the current research investigation on the plant of Cucurbitaceae family , the Physicochemical evaluation and Phytochemical analysis of the plants Cucumis callosus Cucumis prophetarum was conducted. Cucumis callosus and Cucumis prophetarum are well known Indian medicinal plants used in traditional system for various diseases. These comprises of various phytochemical constituents. The extracts of this plant have been pharmacologically evaluated for haematological and gynaecologic disorders anti cancer activity, antioxidant activity, hepatoprotective activity etc. The therapeutic activity is due to the phytochemicals present in the plant. So, it is highly essential to standardize the medicinal plant on the basis of physicochemical and phytochemical aspects to identify the genuine plant part. The results demonstrated that the physico chemical parameters such as ash value, extractive value, foreign matter are the suitable parameters in the standardization of the fruits of Cucumis callosus and Cucumis prophetarum. Thus, it can be assumed that these are the standard parameters in the standardization of fruits of Cucumis callosus and Cucumis prophetarum.