ISSN : 2663-2187

Phytochemical composition and DNA barcoding of Bauhinia scandens leaf extract and its in vitro assessment of antioxidant and anti-inflammatory potential

Main Article Content

Bindu. P. K, Kavya. C. P
» doi: 10.33472/AFJBS.6.4.2024.1019-1045

Abstract

Plants belonging to the genus Bauhinia are gaining more attention in recent years due to the presence of novel phyto compounds. Out of the several species in this genus, Bauhinia scandens is the least explored and thus only very few reports are available with regard to it. Thus, the present investigation focussed on the phytochemical composition of ethanolic extract of B. scandens leaves and the in vitro assessment of antioxidant as well as anti-inflammatory potential of the extract. GC-MS analysis of the ethanol extract revealed the presence of seven major compounds and Benzoic acid, 4-[(trimethylsilyl)oxy]-, trimethylsilyl ester indicated the highest peak value in the extract (peak area: 22.09 %). The presence of flavonoids like vitexins, isoflavones, quercetins, rutins, syringate, gallocatechins, kaempferols, alkaloids, glucosides through multiple gas chromatographic mass spectral analyses demonstrated their pharmacological importance. The phosphomolybdate method was used to analyse the total antioxidant activity of the extract. The EC50 value obtained for antioxidant capacity for the ethanol extract (23.93±0.12 µg/mL) and was comparable with that of the standard (ascorbic acid: 24.73±0.09 µg/mL. The IC50 values of scavenging DPPH radicals for the extract and standard (ascorbic acid) were 39.64±0.02 µg/mL and 37.40 ±0.50 µg/mL, respectively. Strong antioxidant activity of the extract could be attributable to the presence of phenolic compounds. The IC50 values for the extract and standard were 32.22±0.50 µg/mL and 36 ±0.20 µg/mL. Thus this study proved Bauhinia scandens as candidate species in pharmaceutical sector.

Article Details