Medicinal plants play a foremost role in the basic healthcare systems of countries. The therapeutic properties of the plant are determined by the biological substances present in the plant components that are used to produce medicinal remedies. Strychnos colubrina belongs to the Loganiaceace family. It is generally spread in the Chittoor District exactly at Kambakkam hills, Ambakkam, Sadhumalamma kotta, Bramhadevudigundem (Mamandur). Traditionally, plant have been significantly used to treat fever, rheumatism, anthelmintic, cutaneous disorders, and mania. Objective: The current research aimed to explore the phytochemical constituents of the leaves of Strychnos colubrina through phytochemical evaluation, and gas chromatography-mass spectrometry (GC-MS) analysis. Methods: The shade-dried leaves were powdered and extracted with ethanol using the cold maceration method; we conducted a phytochemical examination to evaluate the characteristics of secondary metabolites and used gas chromatography-mass spectrometry (GC-MS) to find the individual phytocompounds in the ethanolic leaf extract. Phytochemicals were determined using molecular weights (m/z) obtained from GC-MS chromatograms. Phytocompounds were identified using the NIST library and spectral peak interpretation.