This study was focused on preparation of AND-SLNs using solvent evaporation method for liver targeting. Characterization of andrographolide was accomplished by using various methods including spectral, chemical, thermal and chromatographic principles. The prepared AND-SLNs were characterized using Zeta, PDI and transmission electron microscopy. The drug-loaded AND-SLNs encapsulation efficiency and behaviour of in-vitro drug release were studied using UV spectroscopy. The in-vitro release study of AND-SLNs compare with the standard drug Silymarin. The release of active moiety andrographolide from the solid lipid nanoparticles showed a biphasic pattern, burst release initial pattern and secondly maintain sustained release. Andrographolide was administered at 500 mg/kg by oral gavages to test its protective effect on hepatic injury biochemically and histopathologically in the blood/liver and liver respectively. In conclusion, andrographolide produced a protective effect against CCl4-induced liver damage. Our study suggests that Andrographolide may be used as a hepato-protective agent against toxic effects caused by CCl4 and other chemical agents in the liver.