To prepare and characterize nanocurcumin and nanopiperine and investigate their insilico interaction against glycosyltransferase (GTFs) of Streptococcus mutans and Lactobacillus rhamnosus. Materials and methodology: Curcumin and Piperine nanoparticles were prepared via sonication and characterized using dynamic light scattering (DLS), UV-Vis spectroscopy, and Fourier-transform infrared (FTIR) spectroscopy. For docking, the molecular structures of the targets and ligands were downloaded from RCSB PDB and PubChem database respectively. The preparation of the target and ligands were done using AutoDock 4.2.6 software. The molecular docking study was then carried out using AutoDock vina and interpreted using BIOVIA Discovery Studio software. Results: DLS revealed an average diameter of 111.2 nm and 110.1 nm for nanocurcumin and nanopiperine, respectively. UV–visible spectra of nanocurcumin and nanopiperine showed characteristic peaks at 425 nm and 350 nm respectively. FTIR spectrum of curcumin and piperine showed peaks corresponding to the functional groups present in the phytocompounds. Docking results demonstrated the efficacy of curcumin and piperine against the glycosyltransferases of S. mutans and L. rhamnosus. Conclusion: Based on the results, we can conclude that, sonication method proves to be an effective and simple approach for nanoparticle preparation of curcumin and piperine. And these phytochemicals are effective against GTFs of S. mutans and L. rhamnosus.