Background: One of the non-Hodgkin's lymphomas (NHL) with the highest incidence rate and making up the vast majority of aggressive lymphoid neoplasms is diffuse large B cell lymphoma. The most prevalent form of NHL in Pakistan is DLBCL Objective: To determine the prevalence of diffuse large b cell lymphoma subgroups by immune-histochemical markers. Methodology: This cross sectional study was carried out at the department of histopathology Fatima memorial hospital, Lahore. The duration was from January 2024 to June 2024. DLBCL was diagnosed using a“panel of lymphoid antibodies that comprised CD20, CD79a, CD3, Ki-67, and Pax5”. On silane-coated slides, slices were mounted for immunohistochemical staining. In order to analyze the data, IBM SPSS version 24 was used. Results: In the current study, a total of 80 patients were enrolled. The male patients were 45 (56.25%) and female were 35 (43.75%). The mean age (SD) in our study was 42 (4.43) years. Out of 80 cases, 37 (46.25%) cases were observed as germinal center while 43 (53.75%) cases were observed as were non-germinal center like subgroup. In our study, 45 (56.25%) cases observed as extranodal while 35(43.75%) cases were observed as nodal lymphoma. Conclusion: Our study concludes that the prevalence of non germinal sub- group of diffuse large B-cell lymphoma is higher than the germinal centre like sub- group.