Background: Hepatitis C Virus (HCV) poses a significant public health challenge globally, with limited data available from specific regions like Muzaffarabad City, Azad Kashmir, Pakistan. This study aimed to evaluate the prevalence of HCV RNA positivity and assess the viral load distribution among the general population in this region. Methods: This retrospective cross sectional study assessed the prevalence and viral load of HCV RNA among 121 participants from the general population of Muzaffarabad City, Azad Kashmir. Data were collected from a local laboratory in the city, where participants had undergone prior HCV RNA testing. Statistical analyses, including descriptive statistics and linear regression, were performed using GraphPad Prism Version 9.3.1. Results: The study found that 26.4% of the participants were HCV RNA positive, with a higher prevalence among females (32.9%) compared to males (15.5%). The viral load varied significantly across the population, with the majority of participants having a viral load of less than 100 copies/ml. However, 17 participants exhibited a viral load exceeding 1,000,000 copies/ml. No significant association (p = 0.9262; R² = 0.000072) was found between age and viral load. Conclusion: Our study shows a significant prevalence of HCV RNA positivity among the general population of Muzaffarabad City, Azad Kashmir, with an overall positivity rate of 26.4%. The data indicates a higher rate of infection among females compared to males. The analysis shows that although viral load differs among age groups, age by itself does not significantly predict viral load. These findings highlight the ongoing challenge of hepatitis C in this region, emphasizing the need for targeted public health strategies to manage and reduce HCV infection rates in the community.