In this article, the biochemical composition of romaine lettuce varieties, i.e. the amount of dry matter, sugar, vitamin C and nitrates contained in it, was analyzed and the yield indicators were presented. When romaine lettuce was grown as a main and repeated crop, the head yield of lettuce from 498.8 to 537.1 g was formed. The highest yield was obtained from Sladkii Khrust (28.2 t/ha), Aktina (29.0 t/ha) and Batsio (30.9 t/ha).