Cadmium (Cd) is a toxic xenobiotic from the heavy metals family. It is primarily accumulated in the liver and kidneys, where it has adverse effects. This study aimed to investigate the protective effect of Nigella sativa oil (NSO) consumption against Cd hepato-nephrotoxicity in rats. Male albino Wistar rats were separated into four groups of 5 animals each and dealt orally with NSO (4%) alone or accompanied by cadmium (50 mg/l) for two months. The results revealed that Cd exposure caused weight loss and increased serum transaminases, uric acid, creatinine, urea, and kidney weight. It significantly increased malondialdehyde (MDA) and carbonyl proteins in both liver and kidneys, while a marked reduction in tissue glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) was observed. Combined treatment with NSO improved body weight, liver and kidney function, reduced oxidative stress, and enhanced the antioxidant potential by reducing MDA and carbonyl proteins. These findings suggest that NSO consumption may protect rats' liver and kidney function against injury caused by Cd; this is achieved by increasing antioxidant enzyme activities and reducing oxidative stress.